Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to TBG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TBG and incubated. A competitive inhibition reaction is launched between TBG (Standards or samples) and Biotin-conjugated TBG with the pre-coated antibody specific for TBG. The more amount of TBG in samples, the less antibody bound by Biotin-conjugated TBG. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TBG in the sample. The color development is stopped and the intensity of the color is measured